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KMID : 0380019930080050457
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Korean Journal of Biotechnology and Bioengineering
1993 Volume.8 No. 5 p.457 ~ p.464
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Effects of Concentration of Glucose and By-product on the Growth of Bacillus amyloliquefaciens 23350
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Abstract
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In this study, an attempt was made to investigate optimum cell growth and products by Bacillus amyloliquefaciens 23350 in batch culture by varing carbon soures. Maximum dry cell density increased with the increase of initial glucose concentration. maximum dry cell density was obtained with the highest value of 5.2g/P at 30g/Q of initial glucose concentration. By adding acetic acid at 20g/P of initial glucose concentration, the cell growth rate decreased with the increase of initial acetic acid concentration. Among the various carbon sources, maximum a--amylase production was obtained with 225unit/ mQ at 10g/e of initial glucose concentration. Optimum production of a-amylase was obtained with 376unit/mL at 2g/P of initial acetic acid concentration and 20g/Q of initial glucose concentration. By 10g/P of initial glucose concentration, both good maximum specific cell growth rate and maximum a_ .amylase production rate were obtained. In view of the results studied optimum production and specific production rate of a-amylase, acetic acid was initially added 2^-4g/Q with 20g/P of initial glucose concentration in batch culture.
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